Our behavior experiment facility is equipped with Ethovision XT (12) software platform to perform behavior tracking and analysis. The behavioral videos are captured with high definition Basler ACE cameras and recorded at a sampling rate of 60 frames per second. The data are also cross checked with manual analysis by the experimenter and validated with analysis performed by personnel blinded to the identity and treatment of the rodents. Genetic manipulation of the receptor is performed by crossing floxed receptor mice with neuronal-Cre lines to obtain brain region specific knockout. Spatial refinement and cell layer specific effects in adult animals are obtained by viral manipulation of gene expression.